a Record control number WOS:000340011900002

a ISSN 0065-1281

a Original cataloging agency NEU GrandLibrary

a Language code of text/sound track or separate title eng

a Classification number QU 300

b Item number .E34 2014

a Personal name Ustunel, Ismail.

h Medium Electronic Document

a Title The effects of water immersion and restraint stress on the expressions of apelin, apelin receptor (APJR) and apoptosis rate in the rat heart

c Statement of responsibility, etc Ismail Ustunel, Nuray Acar, Burcu Gemici, Ozlem Ozbey, Imren Edizer, Hakan Soylu, Filiz Tepekoy, Vecihe Nimet Izgut-Uysal.

a Place of publication, distribution, etc Elsevier ,

c Date of publication, distribution, etc 2014.

a Summary, etc Apelin has been identified as an endogenous ligand of the orphan G-protein-coupled apelin receptor (APJR). These receptors are widely expressed in the central nervous system and periphery and play a role in the regulation of fluid and glucose homeostasis, feeding behavior, vessel formation, cell proliferation and immunity. We aimed to investigate whether water immersion and restraint stress have effects on apelin and APJR expression and apoptosis in heart tissue of male Wistar rats. The cardiac tissues were obtained from control, water immersion and restraint stress (WIRS) and apelin antagonist (F13A)+ WIRS groups of rats and embedded in paraffin wax. Immunohistochemical staining methods were used to localize apelin, APJR and TUNEL immunopositive cells. H-SCORE was used for semi-quantitative determinations. Apelin protein levels were determined by Western blot in the cardiac tissues and plasma corticosteroid levels were measured by enzyme immunoassay (EIA). Apelin immunolocalization was found especially in endothelial cells and mast cells and faintly in cardiomyocytes, APJR immunostaining was shown in endothelial cells and cardiomyocytes, and TUNEL reaction was observed in endothelial cells and in some fibroblasts. Apelin expression was significantly increased in the WIRS and F13A + WIRS groups compared to the control group. The APJR reaction was similar in all groups. The number of TUNEL-positive cells was significantly higher in the F13A + WIRS group than that of the control group. Our study showed that WIRS for 6 h increased plasma corticosterone levels and cardiac apelin expression in rats. The increased levels of apelin inhibited stress-induced apoptosis in heart. These results may be important for the therapeutic approach to a variety of stress-related heart disease. (C) 2013 Elsevier GmbH. All rights reserved.

a Topical term or geographic name as entry element Cell Biology

a Topical term or geographic name as entry element Near East University Article

a Topical term or geographic name as entry element Yakın Doğu Üniversitesi Makale

a Topical term or geographic name as entry element Apelin

a Topical term or geographic name as entry element APJR

a Topical term or geographic name as entry element Water-immersion and restraint stress

a Topical term or geographic name as entry element Pituitary-Adrenal Axis

a Topical term or geographic name as entry element Immunocytochemical Localization

g Relationship information 2014, Vol 116 Issue 5, p675-681

x International Standard Serial Number 00651281

t Title Acta Histochemica

u Uniform Resource Identifier http://library.neu.edu.tr:2048/login?url=http://dx.doi.org/10.1016/j.acthis.2013.12.004